Flicker Vignette - How do I display a spot's intensity?
This vignette shows how do you can display a spot's intensity in the
title bar of the left or right window. The default is to just show the
(x,y) coordinates (raster coordinate system with (0,0) in the upper
left hand corner). The density measurement is taken under the circular
mask. So that needs to be set accordingly.
Enable displaying the grayscale using the (View | Display gray values (C-G)).
This will then change the title display for the selected image
to show for example,
plasmaH.gif (105, 45) 469 tot gray-value
plasmaH.gif (177, 84) 53 mn gray-value
To show the total integrated gray-value, (Quantify | Use sum density else mean density)
To show the mean integrated gray-value, (Quantify | Use sum density else mean density)
To show the circular mask, make sure the two options (View |
Set view measurement options | View measurement circle). Make sure the
(Quantify | List-of-spots else trial-spot measurement-mode (C-J)) is set
to list-of-spots mode. This area is the area of pixel integration
used in computing the integrated density measurement.
To change the new circular mask size, use the measure circle
(diameter) slider. Note: the NxN is one of 1x1, 3x3, 5x5,
..., 51x51 options which is the size of the rectangle enclosing the
As you move the mouse in the selected image, it will display the
data in the image at that position.