Flicker Vignette - How do I set and measure a Region Of Interest (ROI)?

This vignette shows how do you can set and measure a Region Of Interest (ROI). If you have defined the circular mask background density estimate, using C-B, then it will be used to do the background correction for the ROI measurement by subtracting the ROI area*MeanBackgroundDensity.

Method

Setup for the demo

  1. Select the (Quantify | Measure by circle | Capture background (C-B)) or type C-B to capture the background value
  2. Select the (Quantify | Region Of Interest (ROI) | Set ROI ULHC (C-U)) or type C-U to define the upper left hand corner.
  3. Select the (Quantify | Region Of Interest (ROI) | Set ROI LRHC (C-L)) or type C-L to define the lower right hand corner.
  4. Select the (Quantify | Region Of Interest (ROI) | Capture measurement by ROI (C-R)) or type C-R to capture the background corrected density within the ROI.

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Contact us     Flicker is a contributed program available at open2dprot.sourceforge.net/Flicker          Revised: 01/19/2004