Flicker Vignette - How do I warp a gel image's geometry?
This vignette shows how do you can warp one image to the geometry of
the other to make them easier to flicker compare. There are two
warping transforms - affine that requires 3 landmarks and 6 for the
polywarp. (The polywarp is not available yet- [FUTURE]). You first
define the required number of N pairs of corresponding landmarks
between the triangular region you are interested in warping. Then
select the gel you wish to warp. You then apply the warp transform
from the Transform menu. See the example in the Reference
Defining the N landmarks
Select the left or right gel image window by clicking on it. Then
select one of the N landmarks you want to use by clicking on it.
Select the other gel image window by clicking on it. Then select
the coresponding landmark. You may have to Flicker the gels to ensure
they are in fact the correct spots.
Add the landmark to the landmark database by using the
(Landmark | Add landmark (C-A)) or type C-A.
If you do not like the landmark, you can delete the landmark from
the landmark database by using the (Landmark | Delete landmark
(C-D)) or type C-D. You only need to do this step if you
are not happy with the landmark you just created.
Repeats steps  through  until you have the N landmarks.
Warping the image
Select the left or right gel image window you want to warp.
Make sure that you have Allow
transforms checkbox enabled.
Make sure that you have Sequential transforms checkbox
Perform the transform you want to try in the (Transform |
Affine warp) or (Transform | Poly warp) command.
Reposition the transformed image and the other image to the spot
of interest inside of the landmark region.
Enable flickering. It should be now easier to see the
Setup for the demo
for positioning gel images in the left and right scrollable windows