Flicker Vignette - How do I display a spot's intensity?

This vignette shows how do you can display a spot's intensity in the title bar of the left or right window. The default is to just show the (x,y) coordinates (raster coordinate system with (0,0) in the upper left hand corner). The density measurement is taken under the circular mask. So that needs to be set accordingly.

Method

  1. Enable displaying the grayscale using the (View | Checkbox onDisplay gray values (C-G)).
  2. This will then change the title display for the selected image to show for example,
         plasmaH.gif (105, 45) 469 tot gray-value
    or
         plasmaH.gif (177, 84) 53 mn gray-value
  3. To show the total integrated gray-value, (Quantify | Use sum density else mean density) is enabled.
  4. To show the mean integrated gray-value, (Quantify | Use sum density else mean density) is disabled.
  5. To show the circular mask, make sure the two options (View | Set view measurement options | Checkbox onView measurement circle). Make sure the (Quantify | Checkbox onList-of-spots else trial-spot measurement-mode (C-J)) is set to list-of-spots mode. This area is the area of pixel integration used in computing the integrated density measurement.
  6. To change the new circular mask size, use the measure circle (diameter) slider. Note: the NxN is one of 1x1, 3x3, 5x5, ..., 51x51 options which is the size of the rectangle enclosing the circular mask.
  7. As you move the mouse in the selected image, it will display the data in the image at that position.

Setup for the demo

Related vignettes

Contact us     Flicker is a contributed program available at open2dprot.sourceforge.net/Flicker          Revised: 01/19/2004