Flicker Vignette - How do I set and measure a Region Of Interest (ROI)?
This vignette shows how do you can set and measure a Region Of
Interest (ROI). If you have defined the circular mask background
density estimate, using C-B, then it will be used to do the
background correction for the ROI measurement by subtracting the ROI
area*MeanBackgroundDensity.
Method
Setup for the demo
Select the (Quantify | Measure by circle | Capture background
(C-B)) or type C-B to capture the background value
Select the (Quantify | Region Of Interest (ROI) | Set ROI ULHC
(C-U)) or type C-U to define the upper left hand corner.
Select the (Quantify | Region Of Interest (ROI) | Set ROI LRHC
(C-L)) or type C-L to define the lower right hand corner.
Select the (Quantify | Region Of Interest (ROI) | Capture
measurement by ROI (C-R)) or type C-R to capture the
background corrected density within the ROI.