Method

1. Load the two images you want to compare (see vignettes to load gel images if you want different images). One of the gels must be an active clickable map linked to a Web server. You could use, for example, (File | Open demo images | Human Plasma | (Swiss-2DPAGE vs. Merril) gels clickable).
2. Alternative, you can explicitly load one of the over 30 active map gel images in the left or right image. Select the left or right image. Then select the active map image using the (File | Open active map image | ...) command.
3. Flicker align the gels in the region you are interested in.
4. Enable the Click to access DB checkbox. IF flickering was enabled, it will disable flickering.
5. Then select the image with the red "+"'s on spots that are in the active Web database.
6. Click on a red "+" for the spot you are interested in. This will popup a Web browser that links to the spot you have selected in the associated Web database. Each time you click on a spot, it will post the new data in the popup browser window.

Setup for the demo

Related vignettes

• Vignette for loading gel images
• Vignette for loading active map gel images
• Vignette for positioning gel images in the left and right scrollable windows
• Vignette for flicker aligning two gels

Contact us

Flicker is a contributed program available at open2dprot.sourceforge.net/Flicker

Revised: 01/19/2004